Composite

Part:BBa_K4719017:Design

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-03)


phaCAB operon for polyhydroxybutyrate synthesis in K. xylinus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
    Illegal NotI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 642
    Illegal BamHI site found at 3039
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 368
    Illegal NgoMIV site found at 602
    Illegal NgoMIV site found at 914
    Illegal NgoMIV site found at 1193
    Illegal NgoMIV site found at 1606
    Illegal NgoMIV site found at 1673
    Illegal AgeI site found at 341
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We introduced a silent mutation in phaB V181V to ensure that the construct fits RFC1000 assembly standard.


Source

synthetic

References